2026-11-01T22:28:20Z https://nostr.now Thomas Oertner https://nostr.now/npub1pg6t3f8w3mx2yxtsxahtpyenyandm55vuxcy9z46ms9xghma497suysu7n https://fediscience.org/system/accounts/avatars/109/264/507/785/088/048/original/fc284480eb394a1a.jpg https://fediscience.org/system/accounts/avatars/109/264/507/785/088/048/original/fc284480eb394a1a.jpg https://nostr.now/nevent1qqsq8pqyn8lku7gahnxawqy6ajfu67xsf68x52l4fng7rxcjvgc9vvczyq9rfw9ya68vegsewqmkavynxvnkdhwj3nsmqs52htwq5ezl0k5h6sxj3e8 We have tested 20x/1.0 objectives from Zeiss, Leica and Olympus on a new two-photon microscope (980 nm, no cover slip) and the results are - not great. All of these objectives have good IR transmission and are marketed for 2p, but I start to suspect they are still optimized for visible light. This is very disappointing. Objectives with the same NA and correction collar provide much better resolution (at a different price point). Have you used any of these objectives for high resolution 2p imaging? Send me you thoughts, prayers and PSFs, please.<br/> <img src="https://fediscience.org/system/media_attachments/files/113/692/164/735/577/674/original/1afa797ccc05007a.jpg"> <br/> 2024-12-21T18:52:52Z